abstract：:Ruminal proteolysis and subsequent amino acid degradation represent considerable economic loss in ruminant production. The hyper ammonia-producing bacteria (HAB) are largely responsible for amino acid deamination in the rumen. HAB can be controlled with ionophores, but they are also susceptible to antimicrobial plant ...

journal_title：Current microbiology

authors： Flythe M,Kagan I

更新日期：2010-08-01 00:00:00

abstract：:Bt cotton was the first genetically modified crop approved for use in India. However, only a few studies have been conducted to assess the feasibility of its commercial application. Bt cotton is genetically modified to express a proteinaceous endotoxin (Cry) encoded by cry gene of Bacillus thuringiensis that has speci...

journal_title：Current microbiology

authors： Kapur M,Bhatia R,Pandey G,Pandey J,Paul D,Jain RK

更新日期：2010-08-01 00:00:00

abstract：:The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is responsible for nosocomial and community-acquired infections. Hence, rapid and accurate laboratory diagnosis of MRSA is a vital constituent of control measures. The present study evaluated five different methods for the identification of MRSA. A to...

journal_title：Current microbiology

authors： Al-Talib H,Yean CY,Al-khateeb A,Singh KK,Hasan H,Al-Jashamy K,Ravichandran M

更新日期：2010-07-01 00:00:00

abstract：:Genetic engineering of Lactococcus lactis to produce a heterologous protein may cause potential risks to the environment despite the industrial usefulness of engineered strains. To reduce the risks, we generated three auxotrophic recombinant L. lactis subsp. lactis IL1403 strains expressing a heterologous protein, Bmp...

journal_title：Current microbiology

authors： Kim EB,Son JS,Zhang QK,Lee NK,Kim SH,Choi JH,Kang SK,Choi YJ

更新日期：2010-07-01 00:00:00

abstract：:Pseudomonas aeruginosa is a leading cause of hospital-acquired infections and difficult to treat due to acquired-resistance to multiple antibiotics. A pair of strains, M38100A and M38100B, previously identified from a single clinical isolate of P. aeruginosa was investigated to understand phenotypic and genotypic char...

journal_title：Current microbiology

authors： Mahida K,Kwon DH

更新日期：2010-07-01 00:00:00

abstract：:Previously we isolated six actinomycetes strains, 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4, that produce phospholipase D (PLD) with high transphosphatidylation activity. In this study, we identified these strains, and the PLD activities were compared with those of reference strains. 16S rDNA sequences and DNA-DNA hybridi...

journal_title：Current microbiology

authors： Nakazawa Y,Suzuki R,Uchino M,Sagane Y,Kudo T,Nagai T,Sato H,Takano K

更新日期：2010-05-01 00:00:00

abstract：:Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His(6)-tagged fusion proteins in E. coli....

journal_title：Current microbiology

authors： Specht KM,Sheetz KH,Alexander CM,Lamech LT,O'Connor LH,Walker DM,Stevenson HP

更新日期：2010-04-01 00:00:00

abstract：:Genetic studies of Ochrobactrum anthropi, a bacterial species important in bioremediation and biopesticide degradation, are hindered by the lack of suitably regulated gene expression system. A tightly regulated gene-expression system was developed for O. anthropi using the lacI(q) gene and a re-engineered coliphage T5...

journal_title：Current microbiology

authors： Alqublan H,Seleem MN,Boyle SM,Sriranganathan N

更新日期：2010-04-01 00:00:00

abstract：:Endophytes are beneficial microbes that reside intercellularly inside the plants. Interaction of endophytes with the host plants and their function within their host are important to address ecological relevance of endophyte. Four endophytic bacteria OS-9, OS-10, OS-11, and OS-12 were isolated from healthy leaves of O...

journal_title：Current microbiology

authors： Tiwari R,Kalra A,Darokar MP,Chandra M,Aggarwal N,Singh AK,Khanuja SP

更新日期：2010-03-01 00:00:00

abstract：:Cyanide has been proved to be degraded by Klebsiella oxytoca. In order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-DE) electrophoresis approach and MALDI-TOF-MS allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mM cyanid...

journal_title：Current microbiology

authors： Tang P,Hseu YC,Chou HH,Huang KY,Chen SC

更新日期：2010-03-01 00:00:00

abstract：:By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron-sulfur clusters. But so far, there were no articles to confirm the existence of iron-sulfur cluster in RhdA. In this study, R...

journal_title：Current microbiology

authors： Dai Y,Liu J,Zheng C,Wu A,Zeng J,Qiu G

更新日期：2009-11-01 00:00:00

abstract：:A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3(T)) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3(T) was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogeneti...

journal_title：Current microbiology

authors： López NI,Pettinari MJ,Stackebrandt E,Tribelli PM,Põtter M,Steinbüchel A,Méndez BS

更新日期：2009-11-01 00:00:00

abstract：:Recently, we showed that the southern green stink bug (SGSB), Nezara viridula (L.), can transmit Pantoea agglomerans (Ewing and Fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. Here, we hypothesized that our established model could be used to determine if the SGSB was a general, n...

journal_title：Current microbiology

authors： Medrano EG,Esquivel J,Bell A,Greene J,Roberts P,Bacheler J,Marois J,Wright D,Nichols R,Lopez J

更新日期：2009-10-01 00:00:00

abstract：:A novel isolate of a bacterium, capable of degrading trichloroethylene (TCE) and growing on this as the sole carbon source is reported. The test strain was isolated by an enrichment technique with trichloroethylene as the substrate. The isolated strain belongs to the genus Bacillus. The practical utility of cleaning u...

journal_title：Current microbiology

authors： Dey K,Roy P

更新日期：2009-09-01 00:00:00

abstract：:This article outlines a comprehensive analysis of the microbial diversity of aerosols produced during screening in a green waste composting plant using both culture and molecular techniques. Bacteria, thermophilic actinomycetes and fungi were quantified in the aerosols. The structure of the microbial community was exa...

journal_title：Current microbiology

authors： Bru-Adan V,Wéry N,Moletta-Denat M,Boiron P,Delgènes JP,Godon JJ

更新日期：2009-09-01 00:00:00

abstract：:To investigate the correlation between mutations in promoter, attenuator, and the AmpC enzyme overproduction in Escherichia coli. ampC Promoters from 4 Escherichia coli clinical isolates were cloned upstream to the chloramphenicol acetyltransferase (CAT) gene in pCAT3 reporter plasmid. Promoter strengths were measured...

journal_title：Current microbiology

authors： Yu W,Bing L,Zhenhua L

更新日期：2009-09-01 00:00:00

abstract：:Predation by Bdellovibrio-like organisms (BLOs) results in bacterial community succession in aquatic ecosystems. The effects of nutrient loading on the distribution and phylogeny of BLOs remain largely unknown. To this end, we present our findings on BLO diversity from four north-Indian lakes that are variable in thei...

journal_title：Current microbiology

authors： Chauhan A,Fortenberry GZ,Lewis DE,Williams HN

更新日期：2009-07-01 00:00:00

abstract：:Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis...

journal_title：Current microbiology

authors： Tan F,Zhu J,Tang J,Tang X,Wang S,Zheng A,Li P

更新日期：2009-06-01 00:00:00

abstract：:Adenosine 5'-phosphosulfate (APS) reductase is a key enzyme involved in the pathways of sulfate reduction and sulfide oxidation in the biological sulfur cycle. In this study, the gene of APS reductase from Acidithiobacillus ferrooxidans was cloned and expressed in Escherichia coli, the soluble protein was purified by ...

journal_title：Current microbiology

authors： Zheng C,Zhang Y,Liu Y,Wu A,Xia L,Zeng J,Liu J,Qiu G

更新日期：2009-06-01 00:00:00

abstract：:The great increase in the abundance and phylogenetic diversity of Flavobacterium spp. within a few hundred meters downstream of the discharge site of the Westerhöfer Bach, a hardwater rivulet, raised the question whether adjacent soil may serve as a reservoir of bacteria not detected in discharge water. To address thi...

journal_title：Current microbiology

authors： Cousin S

更新日期：2009-05-01 00:00:00

abstract：:Microorganisms that colonize plants require a number of hydrolytic enzymes to help degrade the cell wall. The maize endophyte Acremonium zeae was surveyed for production of extracellular enzymes that hydrolyze cellulose and hemicellulose. The most prominent enzyme activity in cell-free culture medium from A. zeae NRRL...

journal_title：Current microbiology

authors： Bischoff KM,Wicklow DT,Jordan DB,de Rezende ST,Liu S,Hughes SR,Rich JO

更新日期：2009-05-01 00:00:00

abstract：:A Pseudomonas sp. that may be useful in bioremediation projects was isolated from soil. The strain is of potential value because it reduces selenite to elemental red selenium and is unusual in that it was resistant to high concentrations of both selenate and selenite. Exposure of the strain to 50, 100, and 150 mM sele...

journal_title：Current microbiology

authors： Hunter WJ,Manter DK

更新日期：2009-05-01 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) displays important functional diversity in mammalian and plants. So far, however, studies on GAPDH have not included the halotolerant, unicellular green alga Dunaliella salina. In the present study, a GAPDH cDNA was cloned and sequenced from D. salina. It was 1394 bp lo...

journal_title：Current microbiology

authors： Jia Y,Xue L,Liu H,Li J

更新日期：2009-05-01 00:00:00

abstract：:The dicarboxylic acids malate and fumarate increase ruminal pH, reduce methane production, increase propionate and total volatile fatty acid (VFA) production, and reduce lactic acid accumulation in a manner similar to ionophores. These acids stimulate the ruminal bacterium Selenomonas ruminantium to ferment lactate to...

journal_title：Current microbiology

authors： Nisbet DJ,Callaway TR,Edrington TS,Anderson RC,Krueger N

更新日期：2009-05-01 00:00:00

abstract：:The citric acid-producing fungus Aspergillus niger WU-2223L possesses a cyanide-insensitive respiratory pathway catalyzed by alternative oxidase. The regulation of the alternative oxidase under the conditions of citric acid production was determined from the transcription level of the alternative oxidase gene (aox1). ...

journal_title：Current microbiology

authors： Hattori T,Kino K,Kirimura K

更新日期：2009-04-01 00:00:00

abstract：:Salmonella enterica in cattle production systems may be associated with important human and animal disease issues. However, tremendous diversity exists among Salmonella recovered, and more information is needed about strains of greatest potential health concern, particularly those that are multidrug resistant (MDR). B...

journal_title：Current microbiology

authors： Alam MJ,Renter D,Taylor E,Mina D,Moxley R,Smith D

更新日期：2009-04-01 00:00:00

abstract：:To overexpress the chitosanase gene (csn) in F. solani, a vector based on pCAMBIA 1300 was constructed. The csn gene, which is under control of the Aspergillus nidulans gpdA promoter and A. nidulans trpC terminator, was introduced back into the F. solani genome by Agrobacterium tumefaciens-mediated transformation, and...

journal_title：Current microbiology

authors： Liu H,Bao X

更新日期：2009-03-01 00:00:00

abstract：:Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack L-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of L-methioninase (10.78 U/mg protein), rate of methionine uptake (93....

journal_title：Current microbiology

authors： Khalaf SA,El-Sayed AS

更新日期：2009-03-01 00:00:00

abstract：:Exposure to ethanol is a stress condition that Salmonella typhimurium often encounters during its life cycle. Food, beverage, drugs, and cosmetics have a long history of using alcohols to control pathogens. Ethanol is also commonly used for disinfecting medical instruments. This study was conducted to evaluate the eth...

journal_title：Current microbiology

authors： Shoae Hassani A,Malekzadeh F,Amirmozafari N,Hamdi K,Ordouzadeh N,Ghaemi A

更新日期：2009-03-01 00:00:00

abstract：:A gene encoding the rice (Oryza sativa L.) 90-kDa heat shock protein (OsHsp90) was introduced into Escherichia coli using the pGEX-6p-3 expression vector with a glutathione-S-transferase (GST) tag to analyze the possible function of this protein under heat stress for the first time. We compared the survivability of E....

journal_title：Current microbiology

authors： Liu D,Lu Z,Mao Z,Liu S

更新日期：2009-02-01 00:00:00

abstract：:Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsie...

journal_title：Current microbiology

authors： Lakshmi CV,Kumar M,Khanna S

更新日期：2009-01-01 00:00:00

abstract：:We report the in vitro phytopathogen suppression activity of siderophoregenic preparations of Alcaligenes feacalis vis-à-vis the oraganochlorine fungicide, bavistin. Siderophore-rich culture broth, siderophore-rich supernatant, and purified siderophore preparation exerted antifungal activity against Aspergillus niger ...

journal_title：Current microbiology

authors： Sayyed RZ,Chincholkar SB

更新日期：2009-01-01 00:00:00

abstract：:Erwinia amylovora causes fire blight of apple, pear, and other members of the Rosaceae family. The enzyme LuxS catalyzes the last step in the production of autoinducer-2 (AI-2), a molecule implicated with quorum sensing in many bacterial species. It is now well recognized that LuxS also plays a central role in sulfur ...

journal_title：Current microbiology

authors： Gao Y,Song J,Hu B,Zhang L,Liu Q,Liu F

更新日期：2009-01-01 00:00:00

abstract：:A bacterium designated strain B113, able to degrade benzene, toluene, and ethylbenzene compounds (BTE), was isolated from gasoline-contaminated sediment at a gas station in Geoje, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Acinetobacter. The biodegradati...

journal_title：Current microbiology

authors： Kim JM,Jeon CO

更新日期：2009-01-01 00:00:00

abstract：:The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patie...

journal_title：Current microbiology

authors： Cho YS,Schiller NL,Oh KH

更新日期：2008-12-01 00:00:00

abstract：:Culture-based analysis was employed in parallel with PCR amplification of 16S rDNA, coupled with denaturing gradient gel electrophoresis (DGGE), to profile bacterial species associated with different developmental stages of the pine false webworm (PFW), Acantholyda erythrocephala, a sawfly pest responsible for inciden...

journal_title：Current microbiology

authors： Zahner V,Lucarotti CJ,McIntosh D

更新日期：2008-12-01 00:00:00

abstract：:Debaryomyces nepalensis, a halotolerant food-spoiling yeast could grow in complex (YEPD) medium at different pHs ranging between 3.0 and 11.0 in the absence of salt and at pH 3.0-9.0 in the presence of different concentrations of NaCl and KCl. The specific growth rate of D. nepalensis was not affected by the initial p...

journal_title：Current microbiology

authors： Kumar S,Lal P,Gummadi SN

更新日期：2008-12-01 00:00:00

abstract：:In past years, the significance of microbial resistance to biocides has increased. Twenty biocide-resistant bacterial strains were isolated from dental-unit water line biofilm. All strains resisted high biocide concentrations (up to 100 microg ml(-1)): sodium dodecyl sulphate, hydrogen peroxide, sodium hypochlorite, p...

journal_title：Current microbiology

authors： Liaqat I,Sabri AN

更新日期：2008-10-01 00:00:00

abstract：:This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac ...

journal_title：Current microbiology

authors： Beard CE,Court L,Mourant RG,James B,Van Rie J,Masson L,Akhurst RJ

更新日期：2008-09-01 00:00:00

abstract：:From an undisturbed natural forest soil in Ecuador, three fungal strains of the genus Aspergillus were isolated. Based on molecular and morphological features they are described as three new species, named A. quitensis, A. amazonicus, and A. ecuadorensis. ...

journal_title：Current microbiology

authors： Mares D,Andreotti E,Maldonado ME,Pedrini P,Colalongo C,Romagnoli C

更新日期：2008-09-01 00:00:00